A lectin histochemical study was performed to investigate the distribution and changes of the oligosaccharidic component of the glycoconjugates in the lung of chick embryos, of 1-day-old chick, and of the adult animal. For this purpose, a battery of seven horseradish peroxidase-conjugated lectins (PNA, SBA, DBA, WGA, Con A, LTA, and UEA I) were employed. During the first phase of parabronchi and atria formation, D-galactose-(β1→3)-N-acetyl-D-galactosamine, β-N-acetyl-D-galactosamine, D-glucosamine, α-D-mannose, and sialic acid, present at the level of the surface and of cytoplasmic granules of the lining epithelial cells, seem to play a role in regulating morphogenetic phenomena. In the subsequent phases, the parabronchial lumen and the atrial cavities were characterized by the presence of lectin-reactive material rich in terminal D-galactose-( 1/4 -→3)-N-acetyl-D-galactosamine, β-N-acetyl-D-galactosamine, D-glucosamine and α-D-mannose. From day 18 onwards and immediately after hatching, the free border of the cells lining the air capillaries was characterized by the presence of β-N-acetyl-D-galactosamine and α-D-mannose. The appearance of these sugar residues was concomitant with the beginning of respiratory activity.
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/(SICI)1097-4687(200003)243:3<257::AID-JMOR3>3.0.CO;2-M|
|Codice identificativo ISI:||WOS:000085477000003|
|Codice identificativo Scopus:||2-s2.0-0034162661|
|Titolo:||Glycoconjugate sugar residues in the chick embryo developing lung: a lectin histochemical study|
|Appare nelle tipologie:||1.1 Articolo in rivista|