In Sulfolobus solfataricus the binding of the exchange factor 1b (SsEF-1b) to SsEF-1a-GDP displaces the nucleotide and the SsEF-1a-SsEF-1b complex is formed. The complex itself is stable, but it dissociates upon the addition of GDP or Gpp(NH)p but not ATP. Since the rate of the formation of the SsEF-1a-SsEF-1b complex is significatively slower than the rate of the nucleotide exchange catalyzed by SsEF-1b it can be inferred that in vivo the GDP/GTP exchange reaction proceeds via an SsEF-1a/SsEF-1b interaction without involving the formation of a stable binary complex as an intermediate.

The interaction between the archaeal elongation factor 1a and its nucleotide exchange factor 1b

RAIMO, Gennaro;
1999

Abstract

In Sulfolobus solfataricus the binding of the exchange factor 1b (SsEF-1b) to SsEF-1a-GDP displaces the nucleotide and the SsEF-1a-SsEF-1b complex is formed. The complex itself is stable, but it dissociates upon the addition of GDP or Gpp(NH)p but not ATP. Since the rate of the formation of the SsEF-1a-SsEF-1b complex is significatively slower than the rate of the nucleotide exchange catalyzed by SsEF-1b it can be inferred that in vivo the GDP/GTP exchange reaction proceeds via an SsEF-1a/SsEF-1b interaction without involving the formation of a stable binary complex as an intermediate.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11695/9399
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