A battery of six different horseradish peroxidase‐labelled lectins (SBA, DBA, PNA, WGA, ConA and LTA) was used to study the distribution of carbohydrate residues in glycoconjugates during the development of the chick embryo tongue anlage (7th‐21st day of incubation) and in the tongue of 3 day‐old chicken, at the dorsal and ventral epithelium. From the 7th to the 16th day β‐D‐galactose, D‐galactose‐(β1 → 3)‐N‐acetyl‐D‐galactosamine, D‐mannose and β‐N‐acetyl‐D‐glucosamine show the same epithelial distribution at two lingual aspects. From the 17th day to hatching the epithelium of the dorsal and ventral surfaces is characterized by a different distribution of sugar residues. The functional adaptation of the epithelium, as far as the saccharidic moletie distribution is concerned, seems to be completed at the 21st day of incubation.
Use of horseradish peroxidase conjugated lectins for detection of glycoconjugate changes in developing lingual epithelium of the chick embryo
SGAMBATI, Eleonora
1991-01-01
Abstract
A battery of six different horseradish peroxidase‐labelled lectins (SBA, DBA, PNA, WGA, ConA and LTA) was used to study the distribution of carbohydrate residues in glycoconjugates during the development of the chick embryo tongue anlage (7th‐21st day of incubation) and in the tongue of 3 day‐old chicken, at the dorsal and ventral epithelium. From the 7th to the 16th day β‐D‐galactose, D‐galactose‐(β1 → 3)‐N‐acetyl‐D‐galactosamine, D‐mannose and β‐N‐acetyl‐D‐glucosamine show the same epithelial distribution at two lingual aspects. From the 17th day to hatching the epithelium of the dorsal and ventral surfaces is characterized by a different distribution of sugar residues. The functional adaptation of the epithelium, as far as the saccharidic moletie distribution is concerned, seems to be completed at the 21st day of incubation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
