In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria. The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (Km and Vmax values are about 27 WM and 22 nmol min31 mg protein31 and 70 WM and 4 nmol min31 mg protein31, respectively), (b) sensitivity to inhibitors, (c) Ki for the competitive inhibitor phenylsuccinate and (d) pH profiles.
Metabolites transport in isolated yeast mitochondria: fumarate/malate and succinate/malate antiports
PALLOTTA, Maria Luigia;
1999-01-01
Abstract
In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria. The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (Km and Vmax values are about 27 WM and 22 nmol min31 mg protein31 and 70 WM and 4 nmol min31 mg protein31, respectively), (b) sensitivity to inhibitors, (c) Ki for the competitive inhibitor phenylsuccinate and (d) pH profiles.File in questo prodotto:
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