The biocontrol yeast isolates Rhodotorula glutinis LS11, Cryptococcus laurentii LS28 and Aureobasidium pullulans LS30 were tested against Botrytis cinerea and Penicillium expansum on apples artiﬁcially inoculated and stored at 3 and 20 °C. Isolates LS28 and LS30 were most effective, consistently resulting in high reductions of fungal decay, while isolate LS11 was effective only on apples stored at 3 °C. The yeasts showed good in vitro resistance to dicarboximides and copper fungicides, while they were inhibited by triazoles. Isolate LS11, in contrast to LS28 and LS30,was also inhibited by benzimidazoles. The yeastswere tested on naturally-infected apples in semi-commercial conditions for 2 years. They were applied twice: soon after harvesting and 20 days later, at the beginning of the cold storage. The antagonists signiﬁcantly reduced fungal decay when combined with a low dosage of benomyl showing an activity comparable to that exerted by the fungicide alone at full dosage. Periodical monitoring of the epiphytic biocontrol yeast populations in both the ﬁeld and cold room showed a good rate of survival of the antagonists on the skin of treated apples. Speciﬁc ﬁngerprints relying on ampliﬁed restriction length polymorphism (AFLP) were used to integrate the morphology-based monitoring of the yeasts.
|Digital Object Identifier (DOI):||10.1023/A:1023595529142|
|Codice identificativo ISI:||000182580800007|
|Codice identificativo Scopus:||2-s2.0-0038193618|
|Appare nelle tipologie:||1.1 Articolo in rivista|