tThe effects of post-thaw Helium–Neon (He–Ne) laser irradiation on mobility and functionalintegrity of frozen/thawed chicken, pheasant and turkey spermatozoa were investigated.Cytochrome C oxidase (COX) activity was also determined as a measure of the effect ofirradiation on mitochondrial bioenergetics. Semen samples from each species were col-lected, processed and frozen according to the pellet procedure. After thawing, each semensample was divided into two subsamples: the first one was the control; the second onewas irradiated with a single mode continuous He–Ne laser wave (wavelength 632.8 nm;6 mW; 3.96 J/cm2). Then the samples were assessed for sperm mobility (Accudenz®swim-down test), viability (SYBR-14/PI staining), osmotic-resistance (HOS test) and COX activity.The irradiation was effective P < 0.05 increasing sperm motility in the turkey semen(0.228 ± 0.01 compared with 0.294 ± 0.02). The irradiation also caused an increase (P < 0.05)of the COX activity in pheasant (+135 ± 4%) and turkey (+116 ± 4%) sperm, without affectingviability and osmotic-resistance. The COX was positively correlated (P < 0.05) with the via-bility of chicken sperm, however no significant interactions were found between mobilityand COX activity in the three avian species. Due to the difference in energetic metabolismamong avian species used in this study, the He–Ne laser irradiation has a differential actionon bio-stimulation of turkey, chicken and pheasant spermatozoa. The present results are thefirst to elucidate the possibility for restoration of motility of cryopreserved avian spermato-zoa by bio-stimulation provided via He–Ne laser irradiation.
The post-thaw irradiation of avian spermatozoawith He–Ne laser differently affects chicken, pheasantand turkey sperm qualityNS
IAFFALDANO, Nicolaia;PAVENTI, Gianluca;
2013-01-01
Abstract
tThe effects of post-thaw Helium–Neon (He–Ne) laser irradiation on mobility and functionalintegrity of frozen/thawed chicken, pheasant and turkey spermatozoa were investigated.Cytochrome C oxidase (COX) activity was also determined as a measure of the effect ofirradiation on mitochondrial bioenergetics. Semen samples from each species were col-lected, processed and frozen according to the pellet procedure. After thawing, each semensample was divided into two subsamples: the first one was the control; the second onewas irradiated with a single mode continuous He–Ne laser wave (wavelength 632.8 nm;6 mW; 3.96 J/cm2). Then the samples were assessed for sperm mobility (Accudenz®swim-down test), viability (SYBR-14/PI staining), osmotic-resistance (HOS test) and COX activity.The irradiation was effective P < 0.05 increasing sperm motility in the turkey semen(0.228 ± 0.01 compared with 0.294 ± 0.02). The irradiation also caused an increase (P < 0.05)of the COX activity in pheasant (+135 ± 4%) and turkey (+116 ± 4%) sperm, without affectingviability and osmotic-resistance. The COX was positively correlated (P < 0.05) with the via-bility of chicken sperm, however no significant interactions were found between mobilityand COX activity in the three avian species. Due to the difference in energetic metabolismamong avian species used in this study, the He–Ne laser irradiation has a differential actionon bio-stimulation of turkey, chicken and pheasant spermatozoa. The present results are thefirst to elucidate the possibility for restoration of motility of cryopreserved avian spermato-zoa by bio-stimulation provided via He–Ne laser irradiation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
