The lipase VII (from Candida rugosa) activity was studied as a function of the content of pentanol and water in cethyltrimethylammonium bromide (CTAB)/water/pentanol/hexane reverse micelles. The turn-over numbers for the hydrolysis of p-nitrophenyl butyrate (p-NPB) were determined spectrophotometrically. Reverse micelles size and the partition of p-NPB between the aggregates and the oil continuous phase were determined by means of pulsed gradient spin-echo NMR experiments. A strong correlation between enzyme activity and micellar size was found.

Enzymatic activity of lipase entrapped in CTAB/water/pentanol/hexane reverse micelles: A functional and microstructural investigations

LOPEZ, Francesco;CINELLI, Giuseppe;AMBROSONE, Luigi;CEGLIE, Andrea
2004-01-01

Abstract

The lipase VII (from Candida rugosa) activity was studied as a function of the content of pentanol and water in cethyltrimethylammonium bromide (CTAB)/water/pentanol/hexane reverse micelles. The turn-over numbers for the hydrolysis of p-nitrophenyl butyrate (p-NPB) were determined spectrophotometrically. Reverse micelles size and the partition of p-NPB between the aggregates and the oil continuous phase were determined by means of pulsed gradient spin-echo NMR experiments. A strong correlation between enzyme activity and micellar size was found.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11695/48090
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