Identification of genomic regions that have been targets of selection for phenotypic traits is one of the most challenging applications of dense marker panels in animal genetics. In this study, a genomewide scan using approximately 50,000 SNPwas performed in an attempt to identify genomic regions associated with fat deposition in sheep, the importance of this not only being limited to livestock facing future climate changes but also for elucidating the physiology of lipid metabolism. The genotyping results obtained with the OvineSNP50K BeadChip in 2 fat tail breeds were compared with those obtainedin 13 thin tail breeds. Direct sequencing of the annotated genes located in proximity to the markers with opposite allele frequency in thin tail vs. fat tail sheepgave additional SNP of interest. To further confirmthe results of the genomewide scan, we genotyped the SNP within these genes in the 2 groups of sheep. A missense mutation in the BMP2 gene, with different allele frequency in the 2 groups, was detected. Theresults indicated BMP2 and VNRT as the most probable genes involved in the fat tail phenotype.
|Digital Object Identifier (DOI):||http://dx.doi.org/10.2527/jas.2015-9389|
|Codice identificativo ISI:||WOS:000366326100008|
|Codice identificativo Scopus:||2-s2.0-84975515071|
|Titolo:||Signatures of selection identify loci associated with fat tail in sheep|
|Appare nelle tipologie:||1.1 Articolo in rivista|