Molecular ﬁngerprinting of biocontrol agents is pivotal both for environmental monitoring and registration purposes. Fluorescent ampliﬁed fragment length polymorphism (fAFLP) analysis was utilised for the ﬁrst time to investigate the intraspeciﬁc variability of the yeast-like fungus Aureobasidium pullulans, in order to identify speciﬁc molecular markers for its strain LS30, an effective biocontrol agent against major postharvest pathogens on different crops, and to pave the way to the development of molecular-based tools for unequivocal tracking of this agent after its release in the environment. Forty-eight isolates of A. pullulans from phyllosphere and carposphere of several crops from different sites of Southern Italy and Greece were analyzed by using four couples of primers. A pairwise comparison of fAFLP patterns was performed, for each primer pair, by using Dice similarity coefﬁcient (SD). Four matrices were generated and, subsequently, averaged and combined for constructing a single dendrogram, in which clustering of ﬁngerprints was performed with the unweighted pair groups (UPGMA). In the combined dendrogram, most of the isolates grouped into three main fAFLP clusters with levels of similarity ranging from 0.18 to 0.35. Only two isolates (AU73 and AU91) were very similar in all fAFLP patterns. Only primers AC/CA yielded three DNA sized fragments that appeared to be speciﬁc for LS30.
|Digital Object Identifier (DOI):||10.1016/j.postharvbio.2004.05.008|
|Codice identificativo ISI:||000224497100006|
|Codice identificativo Scopus:||2-s2.0-5344224773|
|Appare nelle tipologie:||1.1 Articolo in rivista|