"\"\\\"We investigated in lab-scale experiments the influence of temperature and relative humidity(RH) on the activity of three biocontrol agents - the yeast Metschnikowia pulcherrima LS16 and the. yeast-like fungi Aureobasidium pullulans LS30 and AU34-2 - against infection by A. carbonarius and. OTA accumulation in wine grape berries. The presence of wounds on grape skin dramatically favours. infection of berries by A. carbonarius strain A1102, since unwounded berries showed very low levels. of infection at all conditions of RH and temperature tested. Artificially wounded berries pre-treated with the biocontrol agents were inoculated with the ochratoxigenic A. carbonarius strain A1102 and. were incubated for 5 days at two levels of RH (60% and 100%) and three different temperatures (20, 25 and 30 °C). The three biocontrol agents were all able to prevent infections at 60% R.H and 20 °C. At. 60% RH and 25 °C only strain Au34-2 achieved some protection on day 5, whereas at 30 °C a limited. biocontrol efficacy was shown until day 2 only. At 100% RH, LS16, LS30 and AU34-2 showed an effective protection of grape berries at 20 ° C until the 5th day of incubation. At higher temperatures,. the three biocontrol agents achieved a significant protection only until the 2nd day from the beginning. of the experiment: all the three strains at 25 °C, only strain LS16 at 30 °C. After 5 days, the three biocontrol agents were able to significantly lower contamination of berries with OTA at all conditions. tested, even when protection from infection was not significant, except than at 30 °C and 100% of RH. for all the three strains, and at 25 °C and 100% of RH for strain LS16. These results emphasize the. pivotal influence of environmental factors on the effectiveness of biocontrol against A. carbonarius and. on OTA contamination in wine grape berries, and the need for biocontrol agents that can cope with the. environmental conditions that are more conducive to attack by A. carbonarius.\\\"\""
Environmental factors affect the activity of biocontrol agents against ochratoxigenic Aspergillus carbonarius on wine grape
DE CURTIS, Filippo;IANIRI , GIUSEPPE;DE CICCO, Vincenzo;CASTORIA, Raffaello
2012-01-01
Abstract
"\"\\\"We investigated in lab-scale experiments the influence of temperature and relative humidity(RH) on the activity of three biocontrol agents - the yeast Metschnikowia pulcherrima LS16 and the. yeast-like fungi Aureobasidium pullulans LS30 and AU34-2 - against infection by A. carbonarius and. OTA accumulation in wine grape berries. The presence of wounds on grape skin dramatically favours. infection of berries by A. carbonarius strain A1102, since unwounded berries showed very low levels. of infection at all conditions of RH and temperature tested. Artificially wounded berries pre-treated with the biocontrol agents were inoculated with the ochratoxigenic A. carbonarius strain A1102 and. were incubated for 5 days at two levels of RH (60% and 100%) and three different temperatures (20, 25 and 30 °C). The three biocontrol agents were all able to prevent infections at 60% R.H and 20 °C. At. 60% RH and 25 °C only strain Au34-2 achieved some protection on day 5, whereas at 30 °C a limited. biocontrol efficacy was shown until day 2 only. At 100% RH, LS16, LS30 and AU34-2 showed an effective protection of grape berries at 20 ° C until the 5th day of incubation. At higher temperatures,. the three biocontrol agents achieved a significant protection only until the 2nd day from the beginning. of the experiment: all the three strains at 25 °C, only strain LS16 at 30 °C. After 5 days, the three biocontrol agents were able to significantly lower contamination of berries with OTA at all conditions. tested, even when protection from infection was not significant, except than at 30 °C and 100% of RH. for all the three strains, and at 25 °C and 100% of RH for strain LS16. These results emphasize the. pivotal influence of environmental factors on the effectiveness of biocontrol against A. carbonarius and. on OTA contamination in wine grape berries, and the need for biocontrol agents that can cope with the. environmental conditions that are more conducive to attack by A. carbonarius.\\\"\""I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
