The aim of the present study was to investigate the distribution of the glycoconjugates sialoderivatives in the human testis. Orchidectomy specimens from men aged 18–30 years (Group 1) and from men aged 70–93 years (Group 2) were obtained at autopsy.The studywas performed using digoxigenin-labelled lectins, namely Maackia amurensis II lectin (MAA), Sambucus nigra agglutinin (SNA) and Arachis hypogaea lectin (PNA), in addition to enzymatic and chemical treatments (neuraminidase, KOH–neuraminidase, mild oxidation–neuraminidase, mild oxidation–KOH–neuraminidase, strong oxidation–neuraminidase, strong oxidation–KOH–neuraminidase), to characterise the different glycosidic linkages of the sialoderivatives and to obtain information regarding their structure. In all Group 2 samples, sialic acids linked α-2,3 to galactose and α-2,6 to galactose/N-acetyl-d-galactosamine (Gal/GalNAc), revealed by MAA and SNA, respectively, were observed in testicular interstitial tissue and in the lamina propria. Sialic acid linked α-2,6 to Gal/GalNAcwas detected in only some samples from Group 1. After treatment, PNA showed structural changes and/or the gradual disappearance of sialic acid linked to d-galactose-β(1–3)-N-acetyl-d-galactosamine in testicular components with aging. These findings indicate that changes in the metabolism of sialoderivatives in the testis could be related to morphofunctional changes in various testicular components typical of this organ during aging. This suggests that sialoderivatives are important in the functionality of the mature testis in men, as well as its involution.
|Digital Object Identifier (DOI):||10.1071/RD08292|
|Codice identificativo ISI:||WOS:000266406400001|
|Codice identificativo Scopus:||2-s2.0-67649703523|
|Titolo:||Sialic acid in human testis and changes with ageing|
|Appare nelle tipologie:||1.1 Articolo in rivista|