Forty-nine compounds currently used as additives in foods were tested in combination with three biocontrol agents, the yeasts Rhodotorula glutinis, Cryptococcus laurentii, and the yeastlike fungus Aureobasidium pullulans, to increase their antagonistic activity against Penicillium expansum, the causal agent of blue mold on apples. Twelve additives dramatically improved the antagonistic activity of one or more of the tested biocontrol agents. In a two-way factorial experiment with these selected additives the percentage of P. expansum rots on apples was signiﬁcantly inﬂuenced by the antagonist and the additive as well as by their interaction. The combination of the biocontrol agents and some additives resulted in a signiﬁcantly higher activity with respect to the single treatments applied separately, producing additive or synergistic effects. Some of the selected additives combined with a low yeast concentration (10^6 cells per ml) had comparable or higher efﬁcacy than the biocontrol agents applied alone at a 100-fold higher concentration (10^8 cells per ml). Some organic and inorganic calcium salts, natural gums, and some antioxidants displayed the best results. In general, the effect of each additive was speciﬁc to the biocontrol isolate used in the experiments. Possible mechanisms involved in the activity of these beneﬁcial additives and their potential application in effective formulations of postharvest biofungicides are discussed.
|Codice identificativo ISI:||WOS:000232409600013|
|Codice identificativo Scopus:||2-s2.0-26644457427|
|Titolo:||Integration of Biocontrol agents and food-grade additives for enhancing protection of stored apples from Penicillium expansum|
|Appare nelle tipologie:||1.1 Articolo in rivista|