Abstract: The conformational features of a peptide derived by the 10–30 sequence of the mitochondrial domain of AKAP121 [Ac-1XKKPLALPGMLALLGWWWFFSRKKX25-NH2 (X - Ala)] in water and in a water/triflouroethanol (TFE) mixture at 298 K have been determined by NMR and CD spectroscopy. Backbone clustering analysis of NMR-derived structures led to the identification of a single representative structure in water/TFE. The structure of the peptide consists mainly of an -helix, whose core is the region 7–23, with a less ordered N-terminal part. These data are confirmed by CD analysis. It is noteworthy that the high hydrophobic Trp16–Phe20 segment, that might also mediate interaction with tubulin, is organized in an -helical wheel. Our conformational data can be the starting point for the development of highly selective peptides that interfere with the biological function of the Protein Kinase A scaffold protein AKAP121

Syntethic Peptide Reproducing the Mitochondrial Targeting Motif of AKAP121. Synthesis and Conformational Studies

SAVIANO, Gabriella;
2004

Abstract

Abstract: The conformational features of a peptide derived by the 10–30 sequence of the mitochondrial domain of AKAP121 [Ac-1XKKPLALPGMLALLGWWWFFSRKKX25-NH2 (X - Ala)] in water and in a water/triflouroethanol (TFE) mixture at 298 K have been determined by NMR and CD spectroscopy. Backbone clustering analysis of NMR-derived structures led to the identification of a single representative structure in water/TFE. The structure of the peptide consists mainly of an -helix, whose core is the region 7–23, with a less ordered N-terminal part. These data are confirmed by CD analysis. It is noteworthy that the high hydrophobic Trp16–Phe20 segment, that might also mediate interaction with tubulin, is organized in an -helical wheel. Our conformational data can be the starting point for the development of highly selective peptides that interfere with the biological function of the Protein Kinase A scaffold protein AKAP121
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11695/3016
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