The aim of the research was to determine whether and how the two doses of laser irradiation energy (3.96 and 6.12 J /cm2) can improve the quality characteristics of ram sperm after freeze-thaw process. In this regard semen straws were thawed in water bath at 39oC for 120 seconds. After thawing, semen was divided into three samples: one representing control and the other two were exposed to He-Ne laser irradiation at two different doses of energy (3.96 and 6.12 J /cm2) and mitochondrial activity, cell viability (by flow cytometry), motility and function of plasma membrane integrity test (HOST) were analyzed. He-Ne laser action on thawed ram semen leads to an improvement of motility, viability, mitochondrial function and functional integrity of the membrane for the dose of 6.12 J /cm2, in contrast to the dose of 3.96 J /cm2 which decreases the quality of semen parameters relative to the control sample.

Study on the effects of exposure to different doses of energy generated by a He-Ne laser on the quality of frozen-thawed semen of ram

IAFFALDANO, Nicolaia
Writing – Original Draft Preparation
;
PAVENTI, Gianluca;
2015

Abstract

The aim of the research was to determine whether and how the two doses of laser irradiation energy (3.96 and 6.12 J /cm2) can improve the quality characteristics of ram sperm after freeze-thaw process. In this regard semen straws were thawed in water bath at 39oC for 120 seconds. After thawing, semen was divided into three samples: one representing control and the other two were exposed to He-Ne laser irradiation at two different doses of energy (3.96 and 6.12 J /cm2) and mitochondrial activity, cell viability (by flow cytometry), motility and function of plasma membrane integrity test (HOST) were analyzed. He-Ne laser action on thawed ram semen leads to an improvement of motility, viability, mitochondrial function and functional integrity of the membrane for the dose of 6.12 J /cm2, in contrast to the dose of 3.96 J /cm2 which decreases the quality of semen parameters relative to the control sample.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11695/2062
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