Since the 1950s phthalates (PAEs) are largely used in polymers to enhance the flexibility and extensibility of the materials. Because these compounds are reported to act as endocrine disruptors, and the exposure at high levels can cause harmful effects in the human reproductive system, their determination in food and beverages are fundamental due to their use as food/beverage containers and packaging. Various pretreatment techniques followed by chromatographic analysis have been developed to analyze PAEs from different samples: among them, the dispersive liquid-liquid microextraction (DLLME) is very interesting and powerful. It is based on the addition of an immiscible solvent showing higher density to the aqueous sample for extraction step. In order to concentrate apolar molecules in the dispersed phase it is also essential to add a dispersant solvent which increases the contact between the two immiscible solvents, and having the characteristics to be soluble in both. The interface plays an important role in the extraction process and its development is facilitated by adding just the disperser solvent [1]. This communication would like to show the application of such extraction protocol to different beverage matrices at different alcoholic content (from 5 alc vol-1 to 45 alc vol-1) or recreational beverages (soft drinks, coffee, etc.): using a single extraction protocol followed by GC-IT/MS is possible to analyze DMP, DEP, DBP, BcEP, BBP, DEHP in such matrices. All the analytical parameters will be investigated and discussed. In particular, the role of the vortex step is largely discussed: this step allows to enhance the recoveries (between 95 % and 103 %). On the other hand, Enrichment Factors range between 258 and 300 whereas LODs and LOQs are adequate for determining such compounds at trace and ultra-trace levels in such samples. Further, applications to real samples will be reported. Finally, some comments on the applicability of this methodology to the determination of other organic pollutants will be introduced preliminarily. [1] G. Cinelli, P. Avino, I. Notardonato, A. Centola, M.V. Russo, Anal. Chim. Acta (769 (2013) 72-78.
Phthalates determination in different beverage matrices by dispersive liquid-liquid micro-extraction coupled with GC-IT/MS
RUSSO MV;AVINO P;NOTARDONATO I
2016-01-01
Abstract
Since the 1950s phthalates (PAEs) are largely used in polymers to enhance the flexibility and extensibility of the materials. Because these compounds are reported to act as endocrine disruptors, and the exposure at high levels can cause harmful effects in the human reproductive system, their determination in food and beverages are fundamental due to their use as food/beverage containers and packaging. Various pretreatment techniques followed by chromatographic analysis have been developed to analyze PAEs from different samples: among them, the dispersive liquid-liquid microextraction (DLLME) is very interesting and powerful. It is based on the addition of an immiscible solvent showing higher density to the aqueous sample for extraction step. In order to concentrate apolar molecules in the dispersed phase it is also essential to add a dispersant solvent which increases the contact between the two immiscible solvents, and having the characteristics to be soluble in both. The interface plays an important role in the extraction process and its development is facilitated by adding just the disperser solvent [1]. This communication would like to show the application of such extraction protocol to different beverage matrices at different alcoholic content (from 5 alc vol-1 to 45 alc vol-1) or recreational beverages (soft drinks, coffee, etc.): using a single extraction protocol followed by GC-IT/MS is possible to analyze DMP, DEP, DBP, BcEP, BBP, DEHP in such matrices. All the analytical parameters will be investigated and discussed. In particular, the role of the vortex step is largely discussed: this step allows to enhance the recoveries (between 95 % and 103 %). On the other hand, Enrichment Factors range between 258 and 300 whereas LODs and LOQs are adequate for determining such compounds at trace and ultra-trace levels in such samples. Further, applications to real samples will be reported. Finally, some comments on the applicability of this methodology to the determination of other organic pollutants will be introduced preliminarily. [1] G. Cinelli, P. Avino, I. Notardonato, A. Centola, M.V. Russo, Anal. Chim. Acta (769 (2013) 72-78.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
