Food safety is a goal to which modern societies tend to limit consumer risk of contracting or developing acute or chronic diseases that may occur in humans following intake of residues of xenobiotics present in food and could jeopardize the quality and / or quantity of life of the product. Analytical methods have been developed for the detection of chemicals used in animal husbandry practices to ensure a food safety and to protect the consumers’ health. These methods have been validated according to technical regulations (EC/657/2002), the evaluation of the analytical parameters such as limit of detection (LOD), limit of quantification (LOQ), decision limit (CCα), detection capability (CCβ), accuracy, precision, specificity, selectivity, robustness, reproducibility, repeatability, recovery, etc. .. The methods developed for screening and confirmatory purposes have based on chromatographic separation techniques (gas chromatography and high performance liquid chromatography) and spectroscopic detection systems (UV, FL, DAD) and / or mass spectrometry (ESI, EI, PCI , NCI, APIC, etc.) for the residues detection in eggs (coccidiostats like nicarbazin, robenidine, diclazuril the clazuril, toltrazuril and its metabolites) by means of extraction and purification with divinylbenzene styrene and divinylbenzene styrene columns. The final analysis Multi Reaction Monitoring (MRM) tandem mass spectrometry is performed, in assessing decision limit (CCα) less than 6 mg/kg and detection capability (CCβ) less than 10 mg/kg, for all investigated coccidiostats. Another method has been developed and validated for the detection of residues of anabolic steroids in the urine of cattle and horses. The level of natural amount of endogenous steroids was investigated As part of the present study have been produced some scientific works presented at international conferences and, recently, subjected to peer reviewing by scientific journals.
|Titolo:||Sviluppo di metodi analitici per la ricerca di sostanze chimiche nei prodotti alimentari pericolose per la salute umana|
|Altri titoli:||Development of analytical methods for the search of chemicals in food dangerous for human health|
|Data di pubblicazione:||26-mar-2010|
|Appare nelle tipologie:||8.2 Tesi di dottorato (Ex-ROAD)|