"Epidemiological investigation in chickpea fields of southern Italy. disclosed severe cases of decline. Affected plants showed browning. and necrosis of the crown and main roots while the vegetation. was less vigorous and chlorotic. From symptomatic plant tissues. three fungi were isolated and preliminarily identified as Fusarium. spp. Pathogenicity tests performed on healthy chickpea plants. showed the high virulence of one of the isolates, which reproduced. the field symptoms. The pathogen was re-isolated and showed the. same morphological features of the inoculum, thus fulfilling Koch’s. postulates. The fungal isolate was identified by PCR using specific. primer pairs (ITS1F\/ITS4) designed to amplify a stretch of the. rDNA intergenic spacer 563-573 bp in size, which was sequenced.. The ITS sequence of the new fungal isolate was analyzed by overlaying. it with those of the «International Mycological Association». through the website (www.mycobank.org). The overlay revealed. 99% similarity of the new fungal isolate with F. oxysporum f.sp.. pisi. Additional molecular studies using specific primers (Foc0-12\/. Foc0-12rf), have excluded the possible belonging of the new fungal. isolate to F. oxysporum f.sp. ciceris. According to these results, additional. pathogenicity tests were carried out also on pea and faba. bean plants. The results confirmed the high virulence of the new. isolate of F. oxysporum f.sp. pisi to chickpea and pea, whereas no. symptoms were observed on faba beans. This is the first report of. F. oxysporum f.sp. pisi as a new pathogen of chickpea."

FUSARIUM OXYSPORUM f.sp. PISI CAUSING DECLINE OF CHICKPEA PLANTS IN SOUTHERN ITALY

DE CURTIS, Filippo;VITULLO, Domenico;LIMA, Giuseppe
2013

Abstract

"Epidemiological investigation in chickpea fields of southern Italy. disclosed severe cases of decline. Affected plants showed browning. and necrosis of the crown and main roots while the vegetation. was less vigorous and chlorotic. From symptomatic plant tissues. three fungi were isolated and preliminarily identified as Fusarium. spp. Pathogenicity tests performed on healthy chickpea plants. showed the high virulence of one of the isolates, which reproduced. the field symptoms. The pathogen was re-isolated and showed the. same morphological features of the inoculum, thus fulfilling Koch’s. postulates. The fungal isolate was identified by PCR using specific. primer pairs (ITS1F\/ITS4) designed to amplify a stretch of the. rDNA intergenic spacer 563-573 bp in size, which was sequenced.. The ITS sequence of the new fungal isolate was analyzed by overlaying. it with those of the «International Mycological Association». through the website (www.mycobank.org). The overlay revealed. 99% similarity of the new fungal isolate with F. oxysporum f.sp.. pisi. Additional molecular studies using specific primers (Foc0-12\/. Foc0-12rf), have excluded the possible belonging of the new fungal. isolate to F. oxysporum f.sp. ciceris. According to these results, additional. pathogenicity tests were carried out also on pea and faba. bean plants. The results confirmed the high virulence of the new. isolate of F. oxysporum f.sp. pisi to chickpea and pea, whereas no. symptoms were observed on faba beans. This is the first report of. F. oxysporum f.sp. pisi as a new pathogen of chickpea."
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11695/45859
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