Human breast cancer is the most common invasive cancer and the second most common cause of deaths in women. Since the selenium is able to fight the oxidative damage that is one of the causes driving the cellular damage and is a key factor in the early stage of cancer and in the metastatic process, we recently focused our attention on the selenoproteins involvement in diseases such as liver and breast cancer. In particular, we performed a global analysis of the seleno-transcriptome expression in two human breast cancer cells, MCF-7 and MDA-MB231, compared to human non-cancerous cell line, MCF-10A by quantitative Real time RT-PCR in order to understand how the selenoprotein mRNAs may be differentially expressed in the three different cell lines. We evaluated the expression of 21 genes and the comparison with MCF-10A cells shows that: i) three diodinases (DIO1, DIO2, DIO3), Thioredoxin 2, and two glutathione peroxidases (GPX2 and GPX3) were up-expressed in MCF-7 cells whereas GPX1, GPX5 and GPX7 were down-expressed and ii) only GPX3 was up-expressed in MDA-MB231 cells whereas GPX1, GPX5, GPX6 and GPX7 were down-expressed. Differences resulted also between MCF-7 and MDA-MB231 and this could be due to intrinsic differences between the two human breast cancer cell lines. Indeed, the MCF-7 cells are estrogen-receptor-positive and have wild-type p53 whereas the MDA-MB231 cells are estrogen-receptor-negative and express the mutated p53 gene. In conclusion, in this work we propose a signature of selenoprotein mRNAs specific for human breast cells identifying specific genes for a future estimation of their ability as new breast cancer markers.
|Titolo:||The seleno-transcriptome expression in two human breast cancer cell lines compared to one human non-cancerous breast epithelial cell line|
|Data di pubblicazione:||2015|
|Appare nelle tipologie:||4.2 Abstract in Atti di convegno|