Thirteen simple sequence repeat (SSR) loci were used to screen a collection of Vitis vinifera cultivar 'Tintilia' harvested in Southern Italy and in Spain, as well as other cultivars similar in name and/or in morphological and agronomic characteristics, in order to determine their genetic diversity and relationships. Genetic distances between the 37 accessions analysed have been established showing a close relationship between the Sardinian and Spanish cultivars analysed. Results showed a perfect identity between accessions named 'Tintilia' from Molise, which differed from 'Tintilia' from other Italian regions and from Spain. Furthermore, the molecular data demonstrated that 'Tintilia' and 'Bovale grande' were different varieties, even though they have been reported in the "Vitis International Variety Catalogue" as being identical. The results were compared with a data-set of autochthonous grapes from Campania, confirming the genetic uniqueness of 'Tintilia' towards cultivars from the surrounding area. This work confirms the general applicability of SSR analysis for resolving problems of taxonomic identity.