""The poultry semen cryopreservation is result important both for the value of this technique as fundamental to the practice of artificial insemination, and as an essential tool for the conservation of genetic resources in cryobanks. The choice of the cryoprotectant (CPA) and its concentration is certainly among the most important factor involved in the cryopreservation process.. The packaging of frozen semen in straws would optimize the cryopreservation process by a better identification and safety of frozen semen doses useful particularly in cryobanking. Therefore the aim of this study was to evaluate the effect of two different concentration of dimethylsulfoxide (DMSO) as CPA on the post-thaw quality of turkey semen cryopreserved in straws above the liquid nitrogen vapor.. Seven pools of semen (9-12 ejaculates\\\/pool) were collected from Hybrid Large White toms, an aliquot from each pool was taken for the analysis of fresh semen, and the remaining part of pooled semen was cooled at 5°C for 25 minutes. Each pool was divided into 2 semen samples that were diluted 1:1 (v:v) with the freezing medium composed by Tselutin extender containing DMSO (final concentration of 4% or 10% of DMSO). Thus the semen diluted was aspirated into 0,25 ml plastic straws, equilibrated at 5°C for 20 min and frozen by exposure to liquid nitrogen vapour for 10 min before to be plunged into liquid nitrogen for storage (-196°C). The samples were thawed at 50°C for 10 seconds. Sperm mobility (phase contrast microscopy), viability and osmotic-resistance (SyBr-PI staining) were examined on fresh and post-thawed spermatozoa.. The results obtained showed that the cryopreservation impaired the post-thaw quality of turkey spermatozoa respect to fresh semen. However the quality the frozen semen was affected differently in relation to the DMSO concentration. In fact, the cryopreserved semen quality was better when with the concentration of 10% of DMSO compared to the 4% however only the motility and viability resulted significantly higher (36.92 ± 1.69 vs. 21.92 ± 2.10; 42.09 ± 1.50 vs. 33.48 ± 2.41; P ≤ 0,05) respectively. . In conclusion, these data shows clearly that the higher concentration of penetrating CPAs protect better from cryopreservation damages.. ""

Effects of two different concentrations of DMSO on the quality of turkey semen cryopreserved in straws over liquid nitrogen vapor

IAFFALDANO, Nicolaia;DI IORIO, Michele;ROCCO, MARTINA;MANCHISI, Angelo;
2013-01-01

Abstract

""The poultry semen cryopreservation is result important both for the value of this technique as fundamental to the practice of artificial insemination, and as an essential tool for the conservation of genetic resources in cryobanks. The choice of the cryoprotectant (CPA) and its concentration is certainly among the most important factor involved in the cryopreservation process.. The packaging of frozen semen in straws would optimize the cryopreservation process by a better identification and safety of frozen semen doses useful particularly in cryobanking. Therefore the aim of this study was to evaluate the effect of two different concentration of dimethylsulfoxide (DMSO) as CPA on the post-thaw quality of turkey semen cryopreserved in straws above the liquid nitrogen vapor.. Seven pools of semen (9-12 ejaculates\\\/pool) were collected from Hybrid Large White toms, an aliquot from each pool was taken for the analysis of fresh semen, and the remaining part of pooled semen was cooled at 5°C for 25 minutes. Each pool was divided into 2 semen samples that were diluted 1:1 (v:v) with the freezing medium composed by Tselutin extender containing DMSO (final concentration of 4% or 10% of DMSO). Thus the semen diluted was aspirated into 0,25 ml plastic straws, equilibrated at 5°C for 20 min and frozen by exposure to liquid nitrogen vapour for 10 min before to be plunged into liquid nitrogen for storage (-196°C). The samples were thawed at 50°C for 10 seconds. Sperm mobility (phase contrast microscopy), viability and osmotic-resistance (SyBr-PI staining) were examined on fresh and post-thawed spermatozoa.. The results obtained showed that the cryopreservation impaired the post-thaw quality of turkey spermatozoa respect to fresh semen. However the quality the frozen semen was affected differently in relation to the DMSO concentration. In fact, the cryopreserved semen quality was better when with the concentration of 10% of DMSO compared to the 4% however only the motility and viability resulted significantly higher (36.92 ± 1.69 vs. 21.92 ± 2.10; 42.09 ± 1.50 vs. 33.48 ± 2.41; P ≤ 0,05) respectively. . In conclusion, these data shows clearly that the higher concentration of penetrating CPAs protect better from cryopreservation damages.. ""
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11695/45858
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