"Antioxidant activity (AA) of durum wheat (Triticum durum Desf.) grains was studied using the innovative. LOX\/RNO method, able to simultaneously detect different antioxidant mechanisms, and the TEAC assay,. one of the most widely used assays. Insoluble-bound and free-soluble phenols, hydrophilic and lipophilic. compounds were extracted from eight different whole flour samples; extracts were analyzed for AA and. their content in several antioxidants. The LOX\/RNO method measured very high AA values, with the. highest ones [850e1500 mmol Trolox eq.\/g whole flour (dry weight)] for insoluble-bound phenolic. extracts, highly correlated to total phenolic (r ¼ 0.761, P < 0.001) and ferulic acid (r ¼ 0.816, P < 0.001). contents. Hydrophilic and lipophilic extracts showed lower AA [70-140 and 40e60 mmol Trolox eq.\/g (dry. weight), respectively], highly correlated to flavonoid (r = 0.583, P < 0.01) and protein (r = 0.602,. P < 0.01), as well as b-tocotrienol (r = 0.684, P < 0.05) contents, respectively. Interestingly, the LOX\/RNO. method suggests that insoluble-bound phenolic compounds may exert very strong synergistic interactions. within the extract. Contrarily, the TEAC assay did not correlate to any antioxidant content, resulted. unable to highlight differences among samples, measured much lower AA values and did not suggest. synergism. The use of the LOX\/RNO method is useful to unearth new properties of phytochemicals from. durum wheat grains, potentially giving health benefits.. "
Dissection of antioxidant activity of durum wheat (Triticum durum Desf.) grains as evaluated by the new LOX/RNO method
FRATIANNI, Alessandra;PANFILI, Gianfranco;
2012-01-01
Abstract
"Antioxidant activity (AA) of durum wheat (Triticum durum Desf.) grains was studied using the innovative. LOX\/RNO method, able to simultaneously detect different antioxidant mechanisms, and the TEAC assay,. one of the most widely used assays. Insoluble-bound and free-soluble phenols, hydrophilic and lipophilic. compounds were extracted from eight different whole flour samples; extracts were analyzed for AA and. their content in several antioxidants. The LOX\/RNO method measured very high AA values, with the. highest ones [850e1500 mmol Trolox eq.\/g whole flour (dry weight)] for insoluble-bound phenolic. extracts, highly correlated to total phenolic (r ¼ 0.761, P < 0.001) and ferulic acid (r ¼ 0.816, P < 0.001). contents. Hydrophilic and lipophilic extracts showed lower AA [70-140 and 40e60 mmol Trolox eq.\/g (dry. weight), respectively], highly correlated to flavonoid (r = 0.583, P < 0.01) and protein (r = 0.602,. P < 0.01), as well as b-tocotrienol (r = 0.684, P < 0.05) contents, respectively. Interestingly, the LOX\/RNO. method suggests that insoluble-bound phenolic compounds may exert very strong synergistic interactions. within the extract. Contrarily, the TEAC assay did not correlate to any antioxidant content, resulted. unable to highlight differences among samples, measured much lower AA values and did not suggest. synergism. The use of the LOX\/RNO method is useful to unearth new properties of phytochemicals from. durum wheat grains, potentially giving health benefits.. "I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
