The Italian Podolian is an autochthonous breed belonging to the grey steppe cattle group. In the past, the Podolian was present throughout all of the Adriatic and the southern part of Italy, but now it is restricted to four regions and. displays morphological differences according to. the region. It is thought that the Podolian contributed. heavily to the genetic make-up of the Chianina and Romagnola breeds, while contrasting opinions exist regarding the relationship. between the Podolian and the Piemontese. This paper aimed at characterizing genetic. variability and structure by means of microsatellite loci to assess the relationship. between the Podolian and the most relevant European breeds, and to detect evidence of selection in the considered breeds by investigating the existence of outlier loci. The DNA of. 134 Italian Podolian individuals from 14 different. herds in 4 breeding areas were collected, genotyped at 30 microsatellite loci and compared to 13 Italian and European breeds,including the grey type ones and using N’dama as the outgroup. The Podolian showed the highest values of observed heterozygosity and. polymorphism content. Moreover in a subset of. 50 samples (chosen according to geographic origin), polymorphism data analysis evidenced the absence of a geographically determined. genetic structure. The hierarchical population clustering evidenced a Podolian cluster at K=5. and the phylogenetic tree based on Reynold’s. distances confirmed the genetic links between. the Podolian breeds. Two loci presented significantly. low FST values, suggesting that they could be under the effect of balancing selection.. It can be concluded that Italian Podolian. has no genetic subdivision, contains a notable amount of genetic variability and is closely related to the other Podolian type breeds.
Genetic characterization and structure of the Italian Podolian cattle breed and its relationship with some major European breeds
D'ANDREA, Mariasilvia;MAIORANO, Giuseppe;PILLA, Fabio
2011-01-01
Abstract
The Italian Podolian is an autochthonous breed belonging to the grey steppe cattle group. In the past, the Podolian was present throughout all of the Adriatic and the southern part of Italy, but now it is restricted to four regions and. displays morphological differences according to. the region. It is thought that the Podolian contributed. heavily to the genetic make-up of the Chianina and Romagnola breeds, while contrasting opinions exist regarding the relationship. between the Podolian and the Piemontese. This paper aimed at characterizing genetic. variability and structure by means of microsatellite loci to assess the relationship. between the Podolian and the most relevant European breeds, and to detect evidence of selection in the considered breeds by investigating the existence of outlier loci. The DNA of. 134 Italian Podolian individuals from 14 different. herds in 4 breeding areas were collected, genotyped at 30 microsatellite loci and compared to 13 Italian and European breeds,including the grey type ones and using N’dama as the outgroup. The Podolian showed the highest values of observed heterozygosity and. polymorphism content. Moreover in a subset of. 50 samples (chosen according to geographic origin), polymorphism data analysis evidenced the absence of a geographically determined. genetic structure. The hierarchical population clustering evidenced a Podolian cluster at K=5. and the phylogenetic tree based on Reynold’s. distances confirmed the genetic links between. the Podolian breeds. Two loci presented significantly. low FST values, suggesting that they could be under the effect of balancing selection.. It can be concluded that Italian Podolian. has no genetic subdivision, contains a notable amount of genetic variability and is closely related to the other Podolian type breeds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
