NAD and its derivative NADP mediate hundreds of redox reactions (Enzyme Nomenclature, http://www.chem.qmul.ac.uk/iubmb/enzyme), and thus impact on virtually every metabolic pathway in the living cells. Recent studies show that NAD and NADP play important roles in cell signalling in animal, plants, and fungi (Noctor et al; 2006). NAD(P) is found in mitochondria, chloroplasts, peroxisomes, the cytosol, and other cellular compartments, typically at a total concentration of one to several millimolar (Kasimova et al; 2006). In the light of the fact that mitochondria play a crucial role in integration of major metabolic pathways in plant (Dutilleul et al; 2005), we report a series of experiments testing the hypothesis that mitochondria from tubers of Jerusalem artichoke can synthesize nicotinamide nucleotide from externally added NMN and ATP. The purpose of our work was to verify the presence of mitochondrial NMN-AT (E.C. 2.7.7.1) both in mitochondria isolated from fresh and aged-dehydrated slices tubers of Jerusalem artichoke. References Noctor et al; 2006 J Exp. Bot. V.57. n.8 1603-20; Kasimova et al; 2006 Plant Cell, 18, 3, 688-98; Dutilleul et al; 2005 Plant Physiol. 139, 64-78.
Mitochondria isolated from fresh and aged-dehydrated slices tubers of Jerusalem artichoke can synthesise NAD(P) from NMN externally added
C. DI MARTINO;PALLOTTA, Maria Luigia
2006-01-01
Abstract
NAD and its derivative NADP mediate hundreds of redox reactions (Enzyme Nomenclature, http://www.chem.qmul.ac.uk/iubmb/enzyme), and thus impact on virtually every metabolic pathway in the living cells. Recent studies show that NAD and NADP play important roles in cell signalling in animal, plants, and fungi (Noctor et al; 2006). NAD(P) is found in mitochondria, chloroplasts, peroxisomes, the cytosol, and other cellular compartments, typically at a total concentration of one to several millimolar (Kasimova et al; 2006). In the light of the fact that mitochondria play a crucial role in integration of major metabolic pathways in plant (Dutilleul et al; 2005), we report a series of experiments testing the hypothesis that mitochondria from tubers of Jerusalem artichoke can synthesize nicotinamide nucleotide from externally added NMN and ATP. The purpose of our work was to verify the presence of mitochondrial NMN-AT (E.C. 2.7.7.1) both in mitochondria isolated from fresh and aged-dehydrated slices tubers of Jerusalem artichoke. References Noctor et al; 2006 J Exp. Bot. V.57. n.8 1603-20; Kasimova et al; 2006 Plant Cell, 18, 3, 688-98; Dutilleul et al; 2005 Plant Physiol. 139, 64-78.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
